This MAb reacts with the rod domain of human cytokeratin-19 (CK19), a polypeptide of 40kDa. Its epitope maps between amino acid 312-335. CK19 is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50%-60% of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Flow Cytometry (0.5-1ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1
Western blot 1) Hep-G2 and 2) MCF-7 cell lysateusing of Cytokeratin 19 Mouse Monoclonal Antibody (A53-B/A2.26).
Western Blot Analysis of human Prostate Cancer PC-3 cell lysate Cytokeratin 19 Mouse Monoclonal Antibody (A53-B/A2.26).
SDS-PAGE Analysis Purified Cytokeratin 19 Mouse Monoclonal Antibody (A53-B/A2.26).Confirmation of Integrity and Purity of Antibody.
Immunofluorescence Analysis of MCF-7 cells labeling Cytokeratin 19 with Cytokeratin 19 Mouse Monoclonal Antibody (A53-B/A2.26) followed by Goat anti-mouse IgG-CF488 (Cyan). The nuclear counterstain is Reddot (Red)
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