Binding epitope of this antibody is located in the first EGF-like repeat domain (EGF1) between amino acids 27-59 of Ep-CAM.EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal diluti
Formalin-fixed, paraffin-embed
Formalin-fixed, paraffin-embedded human colon carcinoma stained with EpCAM Mouse Monoclonal Antibody (MOC-31).
Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with EpCAM Mouse Monoclonal Antibody (MOC-31).
Formalin-fixed, paraffin-embedded human endometrial carcinoma stained with EpCAM Mouse Monoclonal Antibody (MOC-31).
Western Blot analysis of human lung tissue lysate using EpCAM Mouse Monoclonal Antibody (MOC-31).
SDS-PAGE Analysis Purified EpCAM Mouse Monoclonal Antibody (MOC-31). Confirmation of Integrity and Purity of Antibody.
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