EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on basolateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Application Notes:
Western Blot (1-2ug/ml), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for
Formalin-fixed, paraffin-embedded human rectal massstained with EpCAM Recombinant Mouse Monoclonal Antibody (rEGP40/1372).
Formalin-fixed, paraffin-embedded human skin tissuestained with EpCAM Recombinant Mouse Monoclonal Antibody (rEGP40/1372).
Western blot analysis of MCF-7 cell lysate using EpCAM Recombinant Mouse Monoclonal Antibody (rEGP40/1372).
SDS-PAGE Analysis PurifiedEpCAM Recombinant Mouse Monoclonal Antibody (rEGP40/1372). Confirmation of Purity and Integrity of Antibody.
Analysis of Protein Array containing more than 19,000 full-length human proteins using EpCAM Monospecific Recombinant Mouse Monoclonal Antibody (rEGP40/1372). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD&apos,s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD&apos,s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Western blot analysis of Human Intestine and HEK293 lysates using EpCAM Mouse Recombinant Monoclonal Antibody (rEGP40/1372).
* VAT and and shipping costs not included. Errors and price changes excepted
