Recognizes a 40-43kDa transmembrane epithelial glycoprotein, identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (EpCAM). EpCAM is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. It is also useful in distinguishing serous carcinomas of the ovary from mesothelioma. This epithelial antigen plays an important role as a tumor-cell marker in lymph nodes from patients with esophageal carcinoma otherwise classified as node-negative. Epithelial antigen has also been suggested as a discriminator between basal cell and baso-squamous carcinomas, and squamous cell carcinoma of the skin.
200ug/ml of Ab purified by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Application Notes:
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 mi
Formalin-fixed, paraffin-embedded human thyroid carcinoma stained with EpCAM Recombinant Rabbit Monoclonal Antibody (EGP40/1555R).
SDS-PAGE Analysis Purified EpCAM Recombinant Rabbit Monoclonal Antibody (EGP40/1555R). Confirmation of Integrity and Purity of Antibody.
Flow Cytometric Analysis of trypsinized-MCF-7 cells. EpCAM Recombinant Rabbit Monoclonal Antibody (EGP40/1555R) followed by goat anti-mouse IgG-CF488 (blue), isotype control (red).
Confocal immunofluorescence analysis of MeOH-fixed HeLa cells. EpCAM Recombinant Rabbit Monoclonal Antibody (EGP40/1555R) followed by goat anti-mouse IgG-CF488 (green). Nuclei counterstained with RedDot
Formalin-fixed, paraffin-embedded human prostate carcinoma stained with EpCAM Recombinant Rabbit Monoclonal Antibody (EGP40/1555R).
Formalin-fixed, paraffin-embedded human colon carcinoma stained with EpCAM Recombinant Rabbit Monoclonal Antibody (EGP40/1555R).
Western Blot Analysis of MCF-7 lysate using EpCAM Rabbit Recombinant Monoclonal Antibody (EGP40/1555R)
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