MAD homolog 9, Madh6, Mothers against decapentaplegic homolog 9, Mothers against DPP homolog 9, SMAD family member 9, SMAD, mothers against DPP homolog 9 (Drosophila), SMAD8A, SMAD8B, Smad9
Smad proteins, the mammalian homologs of the Drosophila mothers against dpp (Mad) have been implicated as downstream effectors of TGFA°a/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MadH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFA°a and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFA°a signaling by interfering with TGFA°a-mediated phosphorylation of other Smad family members.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Flow Cytometry (1-2ug/million cells), ,Immunofluorescence (1-2ug/ml), ,Optimal dilution for a specific application should be determined.
Immunofluorescence Analysis of PFA-fixed HeLa cells stained using SMAD9 Mouse Monoclon
Flow cytometric analysis of PFA-fixed HeLa cells. SMAD9 Mouse Monoclonal Antibody (PCRP-SMAD9-2F4) followed by goat anti-mouse IgG-CF488 (blue), isotype control (red).
Immunofluorescence Analysis of PFA-fixed HeLa cells stained using SMAD9 Mouse Monoclonal Antibody (PCRP-SMAD9-2F4) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).
Immunofluorescence Analysis of PFA-fixed HeLa cells stained using SMAD9 Mouse Monoclonal Antibody (PCRP-SMAD9-2F4)
followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).
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