Myelin basic protein (MBP) is the second most abundant protein in central nervous system (CNS) myelin: it comprises 30% of the total protein and about 10% of the dry weight of myelin. It is the only structural protein found so far to be essential for formation of CNS myelin, and has been called the executive molecule of myelin . MBP can interact with a number of polyanionic proteins including actin, tubulin, calmodulin, and clathrin, and negatively charged lipids, and acquires structure on binding to them. It may act as a membrane actin-binding protein, which might allow it to participate in transmission of extracellular signals to the cytoskeleton in oligodendrocytes and tight junctions in myelin. MBP may be applicable as a marker for oligodendrogliomas. Phosphorylation been shown to decrease its ability to aggregate lipid vesicles and consequently destabilising the compact structure ofmyelin, a destruction that has been observed in demyelinating diseases such as Multiple Sclerosis. MBP/4276 is a useful detector of phosphorylated MBP by binding to Thr98 of human MBP in the phosphorylated state.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
ELISA (For coating, order antibody without BSA), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM T
SDS-PAGE Analysis
Formalin-fixed, paraffin-embedded human brain stained with Myelin Basic Protein Mouse Monoclonal Antibody (MBP/4276).
Formalin-fixed, paraffin-embedded human brain stained with Myelin Basic Protein Mouse Monoclonal Antibody (MBP/4276).
SDS-PAGE Analysis of Purified Myelin Basic Protein Mouse Monoclonal Antibody (MBP/4276). Confirmation of Purity and Integrity of Antibody.
Western Blot Analysis of human brain tissue lysate using Myelin Basic Protein Mouse Monoclonal Antibody (MBP/4276).
Western Blot Analysis of Human Brain, Mouse Brain, Rat Brain, Guinea pig Brain and Hamster Brain tissue lysates using Myelin Basic Protein Mouse Monoclonal Antibody (MBP/4276).
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