Myelin basic protein (MBP) is the second most abundant protein in central nervous system (CNS) myelin: it comprises 30% of the total protein and about 10% of the dry weight of myelin. It is the only structural protein found so far to be essential for formation of CNS myelin, and has been called the executive molecule of myelin . MBP can interact with a number of polyanionic proteins including actin, tubulin, calmodulin, and clathrin, and negatively charged lipids, and acquires structure on binding to them. It may act as a membrane actin-binding protein, which might allow it to participate in transmission of extracellular signals to the cytoskeleton in oligodendrocytes and tight junctions in myelin. MBP may be applicable as a marker for oligodendrogliomas.
200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Application Notes:
Western Blot (2-4ug/ml), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for
SDS-PAGE Analysi
Formalin-fixed, paraffin-embedded human cerebellum stained with MBP Recombinant Mouse Monoclonal Antibody (rMBP/4288).
Formalin-fixed, paraffin-embedded human cerebellum stained with MBP Recombinant Mouse Monoclonal Antibody (rMBP/4288).
SDS-PAGE Analysis of Purified MBP Recombinant Mouse Monoclonal Antibody (rMBP/4288). Confirmation of Purity and Integrity of Antibody.
Western Blot Analysis of human brain tissue lysate using MBP Recombinant Mouse Monoclonal Antibody (rMBP/4288).
Western Blot Analysis of human brain tissue lysate using Myelin Basic Protein Recombinant Mouse Monoclonal Antibody (rMBP/4288).
Western Blot Analysis of Human Brain, Mouse Brain, Rat Brain, Guinea pig Brain and Hamster Brain tissue lysates using Myelin Basic Protein Recombinant Mouse Monoclonal Antibody (rMBP/4288).
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