The human CD146 gene maps to chromosome 11q23 and encodes a trans-membrane glycoprotein, also designated MCAM, MUC 18, that belongs to the immunoglobulin superfamily and functions as a Ca2+-independent cell adhesion molecule. CD146 expression is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage, but not normal melanocytes. CD146 is found on 80% of advanced primary human melanomas and correlates well with development of metastatic disease.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 7.5-8.5, for 45 min at 95°C
Formalin-fixed, paraffin-embedded human Melanoma stained with CD146 Monoclonal Antibody (C146/634)
Formalin-fixed, paraffin-embedded human Tonsil stained with CD146 Monoclonal Antibody (C146/634)
Formalin-fixed, paraffin-embedded human Melanoma stained with CD146 Monoclonal Antibody (C146/634)
Formalin-fixed, paraffin-embedded human Tonsil stained with CD146 Monoclonal Antibody (C146/634)
SDS-PAGE Analysis of Purified MCAM Mouse Monoclonal Antibody (SPM620). Confirmation of Purity and Integrity of Antibody.
SDS-PAGE Analysis of Purified MCAM Mouse Monoclonal Antibody (C146/634). Confirmation of Purity and Integrity of Antibody.
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