MGMT (Marker of Suppressor of Mutation and Carcinogenesis) Antibody, IgG2, Clone: [MGMT/7454], Mouse, Monoclonal
Biozol Catalog Number:
NBT-4255-MSM4-P1
Supplier Catalog Number:
4255-MSM4-P1
Alternative Catalog Number:
NBT-4255-MSM4-P1-100
Manufacturer:
NeoBiotechnologies
Host:
Mouse
Category:
Antikörper
Application:
IHC, WB
Species Reactivity:
Human
Immunogen:
Purified His-tagged MGMT protein
Alternative Names:
6 O methylguanine DNA methyltransferase, Methylated DNA protein cysteine methyltransferase, O 6 methylguanine DNA alkyltransferase, O 6 methylguanine DNA methyltransferase
Cancer chemotherapeutic alkylating agents (e.g. BCNU,) act by inducing formation of lethal cross OOlinks at the O6 OO alkylguanine position in DNA. MGMT transfers alkyl adducts from the O6 OOposition of guanine in DNA (prior to cross OOlink formation) to a cysteine residue in its own sequence, thereby restoring DNA to its intact state. This transfer inactivates the MGMT enzyme and is irreversible, hence the level of MGMT in a cell is directly proportional to the level of DNA OOdamage it can tolerate. In normal tissues, MGMT acts as a suppressor of mutation and carcinogenesis. Tumors with high levels of MGMT are likely to be drug resistant
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Western Blot Analysis of MCF-7, LNCaP, Hep G2 and human liver lysates using MGMT Mouse Monoclonal Antibody (MGMT/7454).
Western blot analysis of Human Liver, Mouse Liver, Rat Liver, Hamster Liver, Guinea pig Liver, Human Pancreas, Mouse Pancreas, Rat Pancreas, Hamster Pancreas, Guinea pig Pancreas, Human skeletal muscle, Mouse skeletal muscle, Rat skeletal muscle, Hamster skeletal muscle and Guinea pig skeletal muscle lysates using MGMT Mouse Monoclonal Antibody (MGMT/7454).
Formalin-fixed, paraffin-embedded human testis stained with MGMT Mouse Monoclonal Antibody (MGMT/7454). Inset: PBS instead of primary antibody, secondary only negative control.
