Recombinant human MTAP protein fragment (aa97-196) (exact sequence is proprietary)
Alternative Names:
BDMF, DMSFH, DMSMFH, Epididymis luminal protein 249, HEL249, LGMBF, MeSAdo phosphorylase, Methylthioadenosine phosphorylase, MSAP, MTA phosphorylase, MTAPase, S-methyl-5-thioadenosine phosphorylase
Recognizes a protein of 31kDa, which is identified as MTAP (5-deoxy-5-methylthioadenosine phosphorylase). It catalyzes the reversible phosphorolysis of methylthioadenosine, which is important in polyamine metabolism and for the salvage of adenine and methionine. The gene encoding MTAP is linked to the tumor suppressor gene, p16INK4A. Deficient levels of MTAP can occur in cancers primarily through co-deletion of the MTAP gene and the p16INK4A gene. Cells expressing MTAP and possessing adenine salvage pathway activity may be less susceptible to malignancy due to growth-inhibitory actions of agents (e.g. antifolates), whose mechanism of action, in part, involves this de novo purine pathway.
200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Application Notes:
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal di
SDS-PAGE Analysis of
Formalin-fixed, paraffin-embedded human Kidney stained with MTAP Recombinant Mouse Monoclonal Antibody (rMTAP/1813).
Formalin-fixed, paraffin-embedded human Kidney stained with MTAP Recombinant Mouse Monoclonal Antibody (rMTAP/1813).
SDS-PAGE Analysis of Purified MTAP Recombinant Mouse Monoclonal Antibody (rMTAP/1813). Confirmation of Purity and Integrity of Antibody.
Western Blot Analysis of Human Brain, Human Heart, Human Kidney, A431 and HeLa lysates using MTAP Recombinant Mouse Monoclonal Antibody (rMTAP/1813).
Western Blot Analysis of HT-29 and HEK-293 cell lysates using MTAP Recombinant Mouse Monoclonal Antibody (rMTAP/1813).
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