In Western blotting, it recognizes proteins in MW range of 265-400kDa, identified as different glycoforms of EMA. This MAb reacts with the DTRP epitope in the tandem repeats. The alpha subunit has cell adhesive properties. It can act both as an adhesion and an anti-adhesion protein. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain, which is involved in cell signaling, through phosphorylations and protein-protein interactions.In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinoma tissues. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver. Class: Monoclonal (Hybridoma model)
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Western Blot (1-2ug/ml), ,Immunohistology (Formalin-fixed) (0.5-1.0ug/ml for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optima
Western Blot Analysis of M
Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with MUC-1 Mouse Monoclonal Antibody (GP1.4).
SDS-PAGE Analysis Purified MUC-1 Mouse Monoclonal Antibody (GP1.4). Confirmation of Integrity and Purity of Antibody
Western Blot Analysis of MCF-7 cell lysate using MUC-1 Mouse Monoclonal Antibody (GP1.4).
Western Blot Analysis of human lung tissue lysate using MUC-1 Mouse Monoclonal Antibody (GP1.4).
Western Blot Analysis of T47D cell lysate using MUC-1 Mouse Monoclonal Antibody (GP1.4).
* VAT and and shipping costs not included. Errors and price changes excepted
