In Western blotting, it recognizes proteins in MW range of 265-400kDa, identified as different glycoforms of EMA. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinomas. Anti-EMA antibody is a useful marker for staining many carcinomas. It stains normal and neoplastic cells from various tissues, including mammary epithelium, sweat glands and colorectal carcinoma. Hepatocellular carcinoma, adrenal carcinoma and embryonal carcinomas are consistently EMA negative, so keratin positivity with negative EMA favors one of these tumors. EMA is frequently positive in meningioma, which can be useful when distinguishing it from other intracranial neoplasms such as schwannomas. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Application Notes:
Western Blot (1-2ug/ml),,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 2
Formalin-fixed, paraffin-embedded human breast carcinoma stained with MUC-1 Recombinant Rabbit Monoclonal Antibody (MUC1/4416R).
Formalin-fixed, paraffin-embedded human breast carcinoma stained with MUC-1 Recombinant Rabbit Monoclonal Antibody (MUC1/4416R).
Western blot analysis of MCF-7 cell lysate using MUC-1 Recombinant Rabbit Monoclonal Antibody (MUC1/4416R).
SDS-PAGE Analysis of Purified MUC-1 Recombinant Rabbit Monoclonal Antibody (MUC1/4416R). Confirmation of Purity and Integrity of Antibody.
Western Blot Analysis of Human kidney and Human Lung tissue lysates using MUC-1 Recombinant Rabbit Monoclonal Antibody (MUC1/4416R).
Western Blot Analysis of T47D cell lysate using MUC-1 Recombinant Rabbit Monoclonal Antibody (MUC1/4416R).
* VAT and and shipping costs not included. Errors and price changes excepted
