This MAb reacts with an extracellular domain (close to transmembrane) of CD56/NCAM. Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. The 135kDa isoform is the basic molecule, which is glycosylated or sialylated to produce the mature species. Anti-CD56 recognizes two proteins of the neural cell adhesion molecule, the basic molecule expressed on most neuroectodermally derived tissues and neoplasms (e.g. retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas, and small cell carcinomas). It is also expressed on some mesodermally derived tumors (rhabdomyosarcoma). Anti-CD56 plays an important role in the diagnosis of nodal and nasal NK/T-cell lymphomas.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Application Notes:
Flow Cytometry (0.5-1ug/ml), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT f
Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with CD56 Rabbit Recombinant Monoclonal Antibody (NCAM1/2217R).
SDS-PAGE Analysis of Purified CD56 Rabbit Recombinant Monoclonal Antibody (NCAM1/2217R). Confirmation of Integrity and Purity of Antibody.
Flow cytometry analysis of lymphocyte-gated PBMCs unstained (gray) or stained with CF488A-labeled CD56 monoclonal antibody (NCAM1/2217R) (green).
Western blot analysis of Human Brain, Mouse Brain and Rat Brain tissue lysates using CD56 Rabbit Recombinant Monoclonal Antibody (NCAM1/2217R).
Western blot analysis of Human Brain tissue lysates using CD56 Rabbit Recombinant Monoclonal Antibody (NCAM1/2217R).
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