Crude neurofilament preparation from porcine spinal cord
Alternative Names:
NEFH, Neurofilament H, Neurofilament Heavy Polypeptide 200kDa, Neurofilament Triplet H Protein, NF-H, NF200
This MAb reacts with a 200kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). It reacts specifically with the phosphorylated KSP/KEP segment at the C-terminus of the heavy subunit (NF-H) of neurofilaments. After dephosphorylation of neurofilaments with alkaline phosphatase, this Ab no longer binds. Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Western Blot (1-2ug/ml), Flow Cytometry (1-2ug/million cells), Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min a
Western Blot Analysis of human B
Formalin-fixed, paraffin-embedded human Cerebellum stained with Neurofilament Mouse Monoclonal Antibody (NE14).
Formalin-fixed, paraffin-embedded Rat Cerebellum stained with Neurofilament Mouse Monoclonal Antibody (NE14).
Western Blot Analysis of human Brain tissue lysate using Neurofilament Mouse Monoclonal Antibody (NE14).
SDS-PAGE Analysis Purified Neurofilament Mouse Monoclonal Antibody (NE14). Confirmation of Integrity and Purity of Antibody
Flow Cytometric Analysis of HEK293 cells using Neurofilament Mouse Monoclonal Antibody (NE14) followed by goat anti-Mouse IgG-CF488 (Blue), Isotype control (Red).
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