Recombinant fragment (around aa185-287) of human NPM1 protein (exact sequence is proprietary)
Alternative Names:
NO38, NPM, NPM1, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Nucleophosmin (nucleolar phosphoprotein B23 numatrin), Nucleophosmin, Nucleophosmin/nucleoplasmin family member 1, Numatrin
Recognizes a 33kDa glycoprotein, identified as Nucleophosmin (NPM). It is predominantly localized in the nucleus of cells in most tissues. NPM is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis. This phosphoprotein moves between the nucleus and the cytoplasm. It is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in exon 12 affecting the C-terminus of the protein are associated with an aberrant cytoplasmic location. Mutations in this gene are associated with acute myeloid leukemia.The antibody may be a useful aid for classification of acute myeloid leukemia.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA), Western Blot (1-2ug/ml),,Immunofluorescence (1-2ug/ml), Flow Cytometry (1-2ug/million cells),,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed
Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with Nucleophosmin-Monospecific Mouse Monoclonal Antibody (NPM1/3287).
SDS-PAGE Analysis Purified Nucleophosmin Mouse Monoclonal Antibody (NPM1/3287). Confirmation of Integrity and Purity of Antibody.
Western Blot Analysis of K562 cell lysate using Nucleophosmin-Monospecific Mouse Monoclonal Antibody (NPM1/3287).
Flow Cytometric Analysis of PFA-fixed HeLa cells using Nucleophosmin-Monospecific Mouse Monoclonal Antibody (NPM1/3287) followed by goat anti-mouse IgG-CF488 (blue), isotype control (red).
Immunofluorescence staining of HeLa cells using Nucleophosmin-Monospecific Mouse Monoclonal Antibody (NPM1/3287) followed by GAM IgG-CF488 (green). Membrane stained with phalloidin (red).
Analysis of Protein Array containing more than 19,000 full-length human proteins using Nucleophosmin-Monospecific Mouse Monoclonal Antibody (NPM1/3287) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD&apos,s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD&apos,s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
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