Recombinant fragment (around aa200-400) of human SERPINA1 protein (exact sequence is proprietary)
Alternative Names:
A1AT, Alpha-1 protease inhibitor, Alpha-1-antiproteinase (Alpha1AT), Dom1, Serine (or cysteine) proteinase inhibitor clade A member 1, Serpin peptidase inhibitor clade A member 1, Short peptide from AAT (SPAAT), Spi1-1
It recognizes a protein of 54kDa, which is identified antitrypsin (AAT). The immunohistochemical staining of AAT is useful in identification of benign and malignant hepatic tumors and yolk sac carcinomas. Positive staining for AAT is also used in detection of benign and malignant lesions of histiocytic nature. This antibody is may also useful tool in the screening of patients with cryptogenic cirrhosis or other forms of liver disease with fibrosis of uncertain origin.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilutio
Formalin-fixed, paraffin-embedded human tonsil stained with Alpha-1-Antitrypsin Mouse Monoclonal Antibody (AAT/6325). Inset: PBS instead of primary antibody, secondary only negative control.
Formalin-fixed, paraffin-embedded human liver in colon stained with Alpha-1-Antitrypsin Mouse Monoclonal (AAT/6325). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.
Analysis of Protein Array containing more than 19,000 full-length human proteins using Alpha-1-Antitrypsin Mouse Monoclonal (AAT/6325). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Western blot analysis of human Lung tissue lysate using SERPINA1 Mouse Monoclonal Antibody (AAT/6325).
Western blot analysis of Human Lung, Mouse Lung, Rat Lung, Hamster Lung and Guinea pig Lung tissue lysates using SERPINA1 Mouse Monoclonal Antibody (AAT/6325).
SDS-PAGE Analysis of Purified Alpha-1-Antitrypsin Mouse Monoclonal (AAT/6325). Confirmation of Purity and Integrity of Antibody.
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