Recombinant Cytochrome C (Mitochondrial Marker) Antibody, Clone: [CYCS/3128R], Rabbit, Monoclonal

Article Name:	Recombinant Cytochrome C (Mitochondrial Marker) Antibody, Clone: [CYCS/3128R], Rabbit, Monoclonal
Biozol Catalog Number:	NBT-54205-RBM6-P0
Supplier Catalog Number:	54205-RBM6-P0
Alternative Catalog Number:	NBT-54205-RBM6-P0-20,NBT-54205-RBM6-P0-100
Manufacturer:	NeoBiotechnologies
Host:	Rabbit
Category:	Antikörper
Application:	FC, IHC, WB
Species Reactivity:	Aves, Canine, Drosophila, Equine, Frog, Human, Mouse, Rat
Immunogen:	Recombinant full-length human CYCS protein
Alternative Names:	CYC, CYCS, HCS, THC4

It recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C, a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This MAb recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e. cytochrome in the mitochondria with heme attached).

Clonality:	Monoclonal
Clone Designation:	[CYCS/3128R]
Molecular Weight:	15kDa
NCBI:	54205
UniProt:	P99999
Form:	200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:	Recombinant Monoclonal Antibody

Application Notes:

Flow Cytometry (1-2ug/million cells), Western Blot (1-2ug/ml), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 9

	Formalin-fixed, paraffin-embedded human Liver stained with Cytochrome C Recombinant Rabbit Monoclonal Antibody (CYCS/3128R).
	SDS-PAGE Analysis Purified Cytochrome C Recombinant Rabbit Monoclonal (CYCS/3128R). Confirmation of Purity and Integrity of Antibody.
	Flow Cytometric Analysis of PFA fixed HeLa cells using Cytochrome C Recombinant Rabbit Monoclonal Antibody (CYCS/3128R) followed by Goat anti-rabbit IgG-CF488 (Blue), Goat anti-mouse IgG-CF488 is Control (Red).
	Western Blot Analysis of Heart tissue lysate using Cytochrome C Recombinant Rabbit Monoclonal Antibody (CYCS/3128R).
	Immunofluorescence Analysis of PFA fixed HeLa cells labeling Cytochrome C Recombinant Rabbit Monoclonal Antibody (CYCS/3128R) followed by Goat anti-rabbit IgG-CF488 (Green)
	Western Blot Analysis of Human Intestine, Human Heart, Human Skeletal Muscle, A549, HeLa and Jurkat lysates using Cytochrome C Recombinant Rabbit Monoclonal Antibody (CYCS/3128R).
	Western Blot Analysis of Heart and Intestine tissue lysates of different species using Cytochrome C Recombinant Rabbit Monoclonal Antibody (CYCS/3128R).