Recombinant fragment (around aa 148-255) of human BOB1 (POU2AF1) protein (exact sequence is proprietary)
Alternative Names:
B cell Oct binding protein 1, B-cell-specific coactivator OBF-1, BOB-1, OBF1, OCA-B, OCAB, OCT-binding factor 1, POU domain class 2-associating factor 1, POU2AF1
BOB.1 expression in a variety of established B-cell lines, representing different stages of B-cell development, has suggested a constitutive, B-cell-specific expression pattern. LP cells in nodular lymphocyte predominant Hodgkin lymphoma, because they are germinal center-derived, are consistently immuno-positive for BOB.1. Conversely, only some cases of classical Hodgkin lymphoma show BOB.1 immuno-reactivity within the Hodgkin and Reed-Sternberg cells.Expression of BOB.1 has been reported in follicular center cell lymphoma, diffuse large B-cell lymphoma and some cases of acute myeloid leukemia.B-CLL, marginal zone lymphoma, and mantle cell lymphoma may show weak to moderate immunoreactivity.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Flow Cytometry (1-2ug/ml), Immunofluorescence (1-2ug/ml),Western Blot (1-2ug/ml),Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for
SDS-PAGE Analysis Purified BOB1
Formalin-fixed, paraffin-embedded human Spleen stained with BOB1 Monospecific Mouse Monoclonal Antibody (BOB1/2424).
Western Blot Analysis of Raji cell lysate using BOB1 Monospecific Mouse Monoclonal Antibody (BOB1/2424).
SDS-PAGE Analysis Purified BOB1 Monospecific Mouse Monoclonal Antibody (BOB1/2424). Confirmation of Purity and Integrity of Antibody.
Flow Cytometric Analysis of paraformaldehyde-fixed Raji cells. BOB1 Monospecific Mouse Monoclonal Antibody (BOB1/2424)., followed by goat anti-Mouse IgG-CF488 (blue) and Isotype Control (Red).
Immunofluorescent staining of paraformaldehyde-fixed Raji cells using BOB1 Monospecific Mouse Monoclonal Antibody (BOB1/2424) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are labeled with RedDot (red).
Analysis of Protein Array containing >19,000 full-length human proteins using BOB1 Monospecific Mouse Monoclonal Antibody (BOB1/2424) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD&apos,s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD&apos,s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
* VAT and and shipping costs not included. Errors and price changes excepted
