B220, CD45R, GP180, Leukocyte common antigen (LCA), Loc, Ly-5, Lyt-4, Protein tyrosine phosphatase receptor type C (PTPRC), Receptor-type tyrosine-protein phosphatase C, T200 glycoprotein
Recognizes a protein of 205kDa-220kDa, identified as CD45RA which is an isoform of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4+ T-cells, 50% of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This MAb is useful in study on the subpopulation of CD4+ or CD8+ T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO +ve) from B cell lymphomas (CD45RA +ve).
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml),Western Blot (1-2ug/ml),Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM ED
Formalin-fixed, paraffin-embedded human tonsil stained with CD45RA Mouse Monoclonal Antibody (111-1C5)
Flow Cytometric Analysis of paraformaldehyde-fixed Raji cells using CD45 Mouse Monoclonal Antibody (111-1C5) followed by Goat anti- Mouse- IgG-CF488 (Blue), Isotype Control (Red).
Paraformaldehyde-fixed Raji cells stained with CD45RA Mouse Monoclonal Antibody (111-1C5) followed by Goat anti-Mouse IgG-CF488 (Green). Counterstain is Phalloidin (red).
SDS-PAGE Analysis of Purified CD45RA Mouse Monoclonal Antibody (111-1C5). Confirmation of Purity and Integrity of Antibody.
Western Blot Analysis of human Spleen tissue lysates using CD45RA Mouse Monoclonal Antibody (111-1C5)
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