Actin is a major component of the cytoskeleton and is present in most cell types. It is highly specific to actin from smooth muscles. This MAb does not stain cardiac or skeletal muscle, however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Flow Cytometry (1-2ug/million cells), ,Immunofluorescence (1-2ug/ml), ,Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for
Formalin-fixed, paraf
Formalin-fixed, paraffin-embedded Rat Lung stained with Smooth Muscle Actin Monoclonal Antibody (1A4 + ACTA2/791).
Formalin-fixed, paraffin-embedded Rat Stomach stained with Smooth Muscle Actin Monoclonal Antibody (1A4 + ACTA2/791).
Formalin-fixed, paraffin-embedded human Leiomyosarcoma stained with Smooth Muscle Actin Monoclonal Antibody (1A4 + ACTA2/791).
Formalin-fixed, paraffin-embedded human Angiosarcoma stained with Smooth Muscle Actin MAb (1A4 + ACTA2/791).
Immunofluorescence Analysis of HeLa cells labeling Smooth Muscle Actin with Smooth Muscle Actin MAb (1A4 + ACTA2/791) followed by Goat anti-Mouse IgG-CF488(Green). The nuclear counterstain is NucSpot (Red).
SDS-PAGE Analysis Purified Smooth Muscle Actin MAb (1A4 + ACTA2/791). Confirmation of Integrity and Purity of Antibody.
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