The human SP100 gene encodes an autoantigen that co-localizes with PML and NDP52 in distinct nuclear domains, called nuclear dots (NDs) or ND10 nuclear bodies. Papova-, adeno-, and herpesviruses begin their transcription and DNA-replication at NDs, which play a role in autoimmunity, viral infections and in the etiology of acute promyelocytic leukemia. SP-100 is an interferon inducible protein that has two splice variants. One splice variant contains a highly conserved copy of the DNA-binding high mobility group 1 protein sequence, and thus represents a novel HMG-box protein. This alternatively spliced variant of SP-100 has a unique expression and localization pattern that is distinct from the SP-100 full-length protein. The SP100 protein is covalently modified by the small ubiquitin-related protein SUMO-1. SP-100 contains a functional nuclear localization signal and an ND-targeting region, which overlaps with the SP-100 homodimerization domain.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
ELISA (For coating, order antibody without BSA), Western Blot (1-2ug/ml), Immunofluorescence (0.5-2ug/ml),,Immunoprecipitation (1-2ug per 100-500ug of total protein (1ml of cell lysate)), Flow Cytometry (0.5-2ug/million cells), ,Immunohistochemistry (For
SDS-PAGE Analysis Purified SP100 Mouse Monoclonal Antibody (PCRP-SP100-1B9). Confirmation of Purity and Integrity of Antibody.
Flow cytometric analysis of PFA-fixed HeLa cells. SP100 Mouse Monoclonal Antibody (PCRP-SP100-1B9) followed by goat anti-mouse IgG-CF488 (blue), unstained cells (red).
Immunofluorescence Analysis of PFA-fixed HeLa cells stained using SP100 Mouse Monoclonal Antibody (PCRP-SP100-1B9) followed by goat anti-mouse IgG-CF488. Membrane stained with phalloidin.
IHC analysis of formalin-fixed, paraffin-embedded human colon. Strong nuclear staining using PCRP-SP100-1B9 at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.
IHC analysis of formalin-fixed, paraffin-embedded human tonsil. Strong nuclear staining using PCRP-SP100-1B9 at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.
Analysis of Protein Array containing more than 19,000 full-length human proteins using SP100 Mouse Monoclonal Antibody (PCRP-SP100-1B9). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD&apos,s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD&apos,s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
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