It recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43 (Workshop III). 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM
Formalin-fixed, paraffin-embedded human Tonsil stained with CD43 Mouse Monoclonal Antibody (84-3C1).
Formalin-fixed, paraffin-embedded human Tonsil stained with CD43 Mouse Monoclonal Antibody (84-3C1).
Immunofluorescence Analysis of K562 cells labeling CD43 with CD43 Monoclonal Antibody (84-3C1) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is NucSpotvÜ.
Western Blot Analysis of K562 cell lysate using CD43 Mouse Monoclonal Antibody (84-3C1).
Flow Cytometric Analysis of Human K562 cells using CD43 Mouse Monoclonal Antibody (84-3C1) followed by Goat anti-Mouse IgG-CF488 (Blue), Isotype control (Red).
SDS-PAGE Analysis of Purified CD43 Mouse Monoclonal Antibody (84-3C1). Confirmation of Integrity and Purity of Antibody.
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