Spectrin is an actin binding protein that is a major component of the plasma membrane skeleton. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Vertebrate spectrins have two alpha-subunits (alpha-I/alpha-II) four beta-subunits (beta-I-beta-IV) and a beta-H subunit creating diversity and specialization of function. Spectrin III is highly expressed in brain, kidney, pancreas and liver, and at lower levels in lung and placenta. Spectrin beta 3 is primarily expressed in nervous tissues with highest expression levels in the cerebellum, where it is found in Purkinje cell soma and dendrites.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA), Flow Cytometry (1-2ug/million cells), (Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixe
SDS-PAGE Analysis
Western Blot Analysis of HeLa and 293 cell lysates using Spectrin beta III Mouse Monoclonal Antibody (SPTBN2/1582).
Formalin-fixed, paraffin-embedded human Pancreas stained with Spectrin beta III Mouse Monoclonal Antibody (SPTBN2/1582).
SDS-PAGE Analysis Purified Spectrin beta III Mouse Monoclonal Antibody (SPTBN2/1582). Confirmation of Integrity and Purity of Antibody.
Western Blot Analysis of human HeLa cell lysate using Spectrin beta III Mouse Monoclonal Antibody (SPTBN2/1582).
Flow Cytometric Analysis of HeLa cells using Spectrin beta III Mouse Monoclonal Antibody (SPTBN2/1582). Goat anti-Mouse IgG-CF488 (Blue), Isotype Control (Red).
Analysis of Protein Array containing more than 19,000 full-length human proteins using Spectrin beta III Mouse Monoclonal Antibody (SPTBN2/1582). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD&apos,s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD&apos,s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of thatMAb to protein X is equal to 29.
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