c fos serum response element binding transcription factor, ELK3, ERP, MCM1, SAP2, Serum response factor (SRF), SRF serum response factor c fos serum response element binding transcription factor
Serum response factor (SRF) is a transcription factor that binds the serum response element (SRE), a sequence that mediates the transient response of many cellular genes to growth stimulation. SRF-binding sites are also constitutive promotor elements in many muscle-specific promotors. At the c-Fos SRE, formation of a ternary complex containing SRF and its accessory protein p62TCF appears to be important for signal transduction. Two related Ets domain proteins, Elk-1 and SRF accessory protein-1 (SAP-1), have DNA binding properties identical to that of p62TCF. Elk-1 and SAP-1 contain two homologous regions of which the two amino-terminal regions, the Ets domain (box A) and the B box, mediate ternary complex formation with SRF. The third homologous region, the C box located toward the C-terminus of the proteins, contains conserved consensus phosphorylation sites for MAP kinases.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
ELISA (For coating, order antibody without BSA), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml),,Immunoprecipitation (1-2ug per 100-500ug of total protein (1ml of cell lysate)), Flow Cytometry (1-2ug/million cells), ,Immunohistochemistry (Formali
SDS-PAGE Analysis Purified SRF Mouse Monoclonal Antibody (PCRP-SRF-1F7). Confirmation of P
Flow cytometric analysis of PFA-fixed HeLa cells. SRF Mouse Monoclonal Antibody (PCRP-SRF-1F7) followed by goat anti-mouse IgG-CF488 (blue), isotype control (red).
SDS-PAGE Analysis Purified SRF Mouse Monoclonal Antibody (PCRP-SRF-1F7). Confirmation of Purity and Integrity of Antibody.
Immunofluorescence Analysis of PFA-fixed HeLa cells stained using SRF Mouse Monoclonal Antibody (PCRP-SRF-1F7) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).
IHC analysis of formalin-fixed, paraffin-embedded human colon. Strong nuclear staining using PCRP-SF7-1F1 at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.
Analysis of Protein Array containing more than 19,000 full-length human proteins using SRF-Monospecific Mouse Monoclonal Antibody (PCRP-SRF-1F7). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD&apos,s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD&apos,s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
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