It recognizes a ~90-95kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190kDa (Workshop IV). This MAb is highly specific to CD71 and shows no cross-reaction with other related proteins. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. CD71/Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml),Optimal dilution for a specific application should be determined.
Flow Cytometric Analysis of human Jurkat cells using CD71
Immunofluorescence Analysis of Human Jurkat cells labeling CD71 with CD71 Mouse Monoclonal antibody (DF1513) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red)
Flow Cytometric Analysis of human Jurkat cells using CD71 Mouse Monoclonal antibody (DF1513) followed by Goat anti-Mouse IgG-CF488 (Orange), cells alone (Blue), Isotype Control (Red).
Western Blot Analysis of Jurkat, Raji, and THP-1 cell lysate using CD71 Mouse Monoclonal antibody (DF1513).
SDS-PAGE Analysis of Purified CD71 Mouse Monoclonal antibody (DF1513). Confirmation of Purity and Integrity of Antibody.
* VAT and and shipping costs not included. Errors and price changes excepted
