It recognizes a ~90-95kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190kDa. This MAb is highly specific to CD71 and shows no cross-reaction with other related proteins. Its epitope is localized in the extracellular domain of CD71. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. CD71/Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Optimal dilution for a specific application should be determined.
Flow Cytometry of human CD71 on Jurkat cells. Black: cells alone, Grey: Isotype Control, Green: CF488-labeled CD71 Monoclonal Antibody (TFRC/1059).
Flow Cytometry of human CD71 on Jurkat cells. Black: cells alone, Grey: Isotype Control, Green: CF488-labeled CD71 Monoclonal Antibody (TFRC/1059)
Flow Cytometric Analysis of PFA-fixed K562 cells using CD71 Mouse Monoclonal Antibody (TFRC/1059) followed by Goat anti-Mouse IgG-CF488 (Blue), Isotype Control (Red).
Immunofluorescence Analysis of K562 cells labeling CD71 with CD71 Mouse Monoclonal Antibody (TFRC/1059) followed by goat anti-mouse IgG-CF488 (green). The nuclear counterstain is RedDot (red).
SDS-PAGE Analysis Purified CD71 Mouse Monoclonal Antibody (TFRC/1059). Confirmation of Purity and Integrity of Antibody.
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