Recombinant human Topoisomerase II alpha fragment (aa1352-1493)
Alternative Names:
ATP hydrolyzing DNA topoisomerase II alfa, DNA gyrase, DNA topoisomerase (ATP hydrolyzing), DNA topoisomerase 2 alpha, DNA topoisomerase II 170kD, DNA topoisomerase II alpha, DNA Topoisomerase2, TOP2A, Topoisomerase DNA II alpha 170kDa, TP2A
It recognizes a 170kDa protein, which is identified as topoisomerase II(TM). It shows no cross-reaction with Topoisomerase II A°a or I. Topo II (TM) plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo II (TM) is also implicated in drug resistance of tumor cells and has been shown to be over-expressed in many human cancers. Decreased expression of Topo IIa is the predominant mechanism of resistance to several chemotherapeutic agents.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Application Notes:
Western Blot (1-2ug/ml),,Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for
Formalin-fixed, paraffin-embedded human tonsil stained with Topo II alpha Recombinant Mouse Monoclonal Antibody (rTOP2A/6569).
Formalin-fixed, paraffin-embedded human lymph node stained with Topo II alpha Recombinant Mouse Monoclonal Antibody (rTOP2A/6569).
IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using rTOP2A/6569 at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.
SDS-PAGE Analysis Purified Topo IIa, Recombinant Mouse Monoclonal Antibody (rTOP2A/6569). Confirmation of Purity and Integrity of Antibody.
Western Blot Analysis of HeLa and Jurkat cell lysates using Topo IIa, Recombinant Mouse Monoclonal Antibody (rTOP2A/6569).
Western Blot Analysis of A549 and Jurkat cell lysates using Topoisomerase II alpha Recombinant Mouse Monoclonal Antibody (rTOP2A/6569).
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