Recognizes a protein of 94kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96). Grp94 shows a high degree of sequence homology with the heat shock protein 90 (hsp90). This MAb is highly specific to grp94 and shows minimal cross-reaction with other members of the HSP90 family. Grps are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Grp94 has been briefly studied as a prognostic factor in breast cancer.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM
Immunofluorescence Analysis
Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with GRP94 Rat Monoclonal Antibody (9G10.F8.2).
Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with GRP94 Rat Monoclonal Antibody (9G10.F8.2).
Immunofluorescence Analysis of HePG2 cells labeling GRP94 with GRP94 Rat Monoclonal Antibody (9G10.F8.2) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot.(Red).
Flow Cytometric Analysis of PFA-fixed HePG2 cells using GRP94 Rat Monoclonal Antibody (9G10.F8.2) followed by Goat anti-Rat- IgG-CF488 (Blue), Isotype Control (Red).
SDS-PAGE Analysis of Purified GRP94 Rat Monoclonal Antibody (9G10). Confirmation of Purity and Integrity of Antibody.
SDS-PAGE Analysis of PurifiedRTM1 Rat Monoclonal Antibody (9G10.F8.2). Confirmation of Purity and Integrity of Antibody.
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