GAP-modifying protein 1, GMP1, OFC10, PIC1, SENP2, Sentrin 1, Small ubiquitin-related modifier 1, SMT3, SMT3 suppressor of mif two 3 homolog 1, SMT3C, SMT3H3, Ubiquitin homology domain protein PIC1, Ubiquitin Like 1, Ubiquitin like protein UBL1
This MAb is specific to SUMO-1 and shows no cross-reaction with either SUMO-2 or SUMO-3. The small ubiquitin-related modifier (SUMO) proteins, which include SUMO-1, SUMO-2 and SUMO-3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursor proteins that undergo processing before conjugation to target proteins. Also, both utilize the E1, E2, and E3 cascade enzymes for conjugation. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins to a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. The unconjugated SUMO-1 protein localizes to the nuclear membrane.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM
Western Blot of HeLa cell lysate using SUMO1 Mouse Monoclonal Antibody (SM1/495)
Flow Cytometric Analysis of paraformaldehyde-fixed HepG2 cel
Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with SUMO-1 Mouse Monoclonal Antibody (SM1/495)
Western Blot of HeLa cell lysate using SUMO1 Mouse Monoclonal Antibody (SM1/495)
Flow Cytometric Analysis of paraformaldehyde-fixed HepG2 cells using SUMO-1 Mouse Monoclonal Antibody (SM1/495) followed by goat anti- Mouse- IgG-CF488 (Blue), Isotype Control (Red).
Paraformaldehyde-fixed HepG2 cells stained with SUMO-1 Mouse Monoclonal Antibody (SM1/495)followed by goat anti-Mouse IgG-CF488 (Green). Cell membranes are stained with Phalloidin (red).
SDS-PAGE Analysis of Purified SUMO-1 Mouse Monoclonal Antibody (SM1/495). Confirmation of Purity and Integrity of Antibody.
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