Focal adhesions are identified as areas within the plasma membrane of tissue culture cells that adhere tightly to the underlying substrate. In vivo, these regions are involved in the adhesion of cells to the extracellular matrix. Paxillin and vinculin are cytoskeletal, focal adhesion proteins that are components of a protein complex which links the Actin network to the plasma membrane. Vinculin binding sites have been identified on other cytoskeletal proteins, including Talin and (TM)-Actinin. In addition, vinculin, Talin and (TM)-Actinin each contain Actin binding sites. Expression of vinculin and Talin have been shown to be affected by the level of Actin expression. (TM)-Actinin has been shown to link Actin to integrins in the plasma membrane through interactions with the vinculin and Talin complex or by a direct interaction with integrin.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Formalin-fixed, paraffin-embedded human pancreas
Formalin-fixed, paraffin-embedded human placenta stained with Vinculin Recombinant Rabbit Monoclonal Antibody (VCL/7091R) at 2ug/ml. Inset: PBS instead of primary antibody, secondary only negative control.
Formalin-fixed, paraffin-embedded human pancreas stained with Vinculin Recombinant Rabbit Monoclonal Antibody (VCL/7091R) at 2ug/ml.
SDS-PAGE Analysis of Purified Vinculin Recombinant Rabbit Monoclonal Antibody (VCL/7091R). Confirmation of Purity and Integrity of Antibody.
Western Blot Analysis of Skeletal muscle tissue lysates of different species using Vinculin Recombinant Rabbit Monoclonal Antibody (VCL/7091R).
Western Blot Analysis of Human Skeletal muscle, A431, HeLa and HEK293 lysates using Vinculin Recombinant Rabbit Monoclonal Antibody (VCL/7091R).
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