Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA), ,Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml). ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heati
Flow Cytometric Analysis of Jurkat cells. CD3e Mouse Monoclonal Antibody (UCHT1) followed by goat anti-mouse IgG-CF488 (blue), isotype control (red).
Immunofluorescence Analysis of Jurkat cells labeling CD3e. CD3e Mouse Monoclonal Antibody (UCHT1) followed by goat anti-mouse IgG-CF488 (green). The nuclear counterstain is RedDot (red).
Immunofluorescence Analysis of Jurkat cells labeling CD3e. CD3e Mouse Monoclonal Antibody (UCHT1) followed by goat anti-mouse IgG-CF488 (green). The nuclear counterstain is RedDot (red).
Flow cytometric analysis of lymphocyte-gated PBMCs. Cells stained using CD3 Mouse Monoclonal Antibody (UCHT1) (red). Isotype control (gray) followed by goat anti-mouse CF640R.
Western Blot analysis of Jurkat cell lysate using CD3e Mouse Monoclonal Antibody (UCHT1)
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