Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. It is found on all mature T-lymphocytes, NK, T-cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation. It is expressed, typically at high levels, on peripheral T cells and majority of T cell neoplasms. Thymocytes express CD3 at different level on the cell surface in the course of differentiation and, in cortical thymus, CD3 is predominantly Intracytoplasmic. This MAb is particularly useful for induction of T cell activation in vitro and in vivo due to its unusual IgG3 isotype.
200ug/ml of Ab Purified from rabbit anti-serum by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Functional Studies (Order Ab without Azide),Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Optimal dilution for a specific application should be determined.
Immunofluorescence Analysis of Jurkat cells labeling CD3e with CD3e Mouse Monoclonal Antibody (RIV9)
Flow Cytometric Analysis of Jurkat cells using CD3e Mouse Monoclonal Antibody (RIV9) followed by Goat anti-Mouse IgG-CF488 (Blue), Isotype Control (Red).
Immunofluorescence Analysis of Jurkat cells labeling CD3e with CD3e Mouse Monoclonal Antibody (RIV9) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).
SDS-PAGE Analysis CD3e Mouse Monoclonal Antibody (RIV9). Confirmation of Integrity and Purity of Antibody.
Flow cytometry analysis of lymphocyte-gated PBMCs unstained (gray) or stained with CF488A-labeled CD3 mouse monoclonal antibody (RIV9) (green)
Immunofluorescence Analysis of methanol-fixed human tonsil cryosection stained with CF488A CD3e clone RIV9 (green), mounted in EverBrite Mounting Medium with DAPI (nuclei, blue).
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