Cluster of differentiation 3 (CD3) is a multimeric protein complex, known historically as the T3 complex, and is composed of four distinct polypeptide chains, epsilon ( ), gamma (A), delta ( OOL) and zeta ( OOEÖ), that assemble and function as three pairs of dimers ( A, OOL, OOEÖ OOEÖ). The CD3 complex serves as a T cell co-receptor that associates noncovalently with the T cell receptor (TCR).CD3 antigen appearance at all stages of T cell development, and makes it an ideal T cell marker for both the detection of normal T cells and T cell neoplasms (lymphomas and leukemias).
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Application Notes:
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal diluti
IHC analysis of formalin-fixed, paraffin-embedded human bra
Formalin-fixed, paraffin-embedded human tonsil stained with CD3e Recombinant Rabbit Monoclonal Antibody (CD3e/4653R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.
IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using C3e/4653R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.
Western Blot Analysis of human Jurkat cell lysate using CD3e Recombinant Rabbit Monoclonal Antibody (CD3e/4653R).
Western Blot Analysis of Human Spleen tissue lysate using CD3e Recombinant Rabbit Monoclonal Antibody (C3e/4653R).
SDS-PAGE Analysis of Purified CD3e Recombinant Rabbit Monoclonal Antibody(C3e/4652R ). Confirmation of Purity and Integrity of Antibody
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