CD19 is a transmembrane glycoprotein that contains two extracellular immunoglobulin-like domains. CD19 is present in both benign and malignant B-cells and is considered to be the most reliable surface marker of this lineage over a wide range of maturational stages. In normal lymphoid tissue, CD19 is observed in germinal centers, in mantle zone cells, and in scattered cells of the inter-follicular areas. Anti-CD19 exhibits an overall immunoreactivity pattern similar to those of the antibodies against CD20 and CD22. However, in contrast to CD20, expression of CD19 is continuous throughout B-cell development and through terminal differentiation of B-cells into plasma cells. Anti-CD19 positivity is seen in the vast majority of B-cell neoplasms commonly at a lower intensity than normal B-cell counterparts. Plasma cell neoplasms are nearly always negative, as are T-cell neoplasms.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Application Notes:
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution
SDS-PAGE Ana
Formalin-fixed, paraffin-embedded human lymph node stained with CD19 Recombinant Mouse Monoclonal Antibody (rCD19/4591).
Formalin-fixed, paraffin-embedded human lymph node stained with CD19 Recombinant Mouse Monoclonal Antibody (rCD19/4591).
SDS-PAGE Analysis of Purified CD19 Recombinant Mouse Monoclonal Antibody (rCD19/4591). Confirmation of Integrity and Purity of Antibody
Western blot analysis of Raji cell lysate using CD19 Recombinant Mouse Monoclonal Antibody (rCD19/4591).
Western blot analysis of Human Tonsil tissue lysates using CD19 Recombinant Mouse Monoclonal Antibody (rCD19/4591).
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