p21/WAF1, also known as cip1 and cyclin-dependent kinase interacting protein 1, is a 21kDa protein that belongs to the CDI family of cyclin-dependent kinase inhibitors. In humans p21/WAF1 is encoded by the CDKN1A gene, the transcription of which is regul
Each vial contains 100 µg 1mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
Source:
WA1 is a mouse IgG1 monoclonal antibody derived from the fusion of SP2 mouse myeloma cells with splenocytes from a BALB/c mouse immunized with human p21 protein.
Formula:
Each vial contains 100 µg 1mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
Application Notes:
The WA1 antibody is suitable for the detection of p21/WAF1 by flow cytometry, immunocytochemistry, ELISA and western blotting. The antibody is also suitable for the detection of p21/WAF1 by immunohistochemistry of frozen and paraffin embedded tissues. Paraffin embedded tissues require pre-treatment prior to staining with the WA1 antibody. Antigen retrieval by heat treatment using sodium citrate buffer is suggested for this purpose. Optimal antibody dilutions for the different applications should be determined by titration.
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