A portion of amino acids 115-144 from the human protein was used as the immunogen for this AKT1 antibody.
AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. It and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50
Application Notes:
The stated application concentrations are suggested starting amounts. Titration of the AKT1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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