A portion of amino acids 424-453 from the human protein was used as the immunogen for this BRAF antibody.
BRAF is a protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway. [UniProt]
Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50
Application Notes:
The stated application concentrations are suggested starting amounts.Titration of the BRAF antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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