A portion of amino acids 62-91 from the human protein was used as the immunogen for this anti-GAPDH antibody.
GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains.
Western blot: 1:500-1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50
Application Notes:
The stated application concentrations are suggested starting amounts. Titration of the anti-GAPDH antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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