KLH conjugated synthetic peptide cYPYDVPDYA was used as the immunogen for the anti-HA antibody.
Epitope tags consisting of short sequences recognized by well-characterizated monoclonal antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYA) and Myc Tag (AEEQKLISEEDLLRKRREQLKHKLE), recognized by monoclonal antibody clones 12CA5 and 9E10, respectively, are illustrative examples. NSJs anti-HA monoclonal antibody (Clone 12CA5) provides a simple solution to detect the expression of an HA-tagged protein in cells.
Clonality:
Monoclonal
Clone Designation:
[12CA5]
Purity:
Purified
Form:
In 1X PBS, pH 7.4, with 0.09% sodium azide
Antibody Type:
Primary Antibody
Application Dilute:
Western blot: 1:1000,Immunoprecipitation: published application
Application Notes:
Titration of the anti-HA antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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