Purified recombinant HIS-tagged fusion protein and poly-HIS peptide were used to produced this monoclonal anti-His antibody.
Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH), recognized by the monoclonal antibody clone 6AT18, provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified from cell lysates by affinity chromatography using Nickel-Sepharose resin. NSJBios anti-6xHIS monoclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins in cells.
Clonality:
Monoclonal
Clone Designation:
[6AT18]
Purity:
Purified
Form:
In 1X PBS, pH 7.4, with 0.09% sodium azide
Antibody Type:
Primary Antibody
Application Dilute:
Western blot: 1:1000-1:4000
Application Notes:
The stated application concentrations are suggested starting amounts. Titration of the anti-His antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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