Purified recombinant fusion protein was used to produced this monoclonal GST antibody.
Glutathione S-transferase (GST) was originally cloned from parasite Schistosoma japonicum and it is now a widely used protein fusion partner. Vectors containing GST Tag have been developed for both prokaryotic and eukaryotic systems. The GST fusion proteins are easily purified from cell lysates by affinity chromatography using Glutathione Sepharose 4B to elute out the GST fusion protein from the column with a denaturing form of glutathione. Using the NSJBio anti-GST antibody provides a simple solution to detect the expression of GST fusion proteins in cells.
Clonality:
Monoclonal
Clone Designation:
[21CT54.13.1]
Purity:
Purified
Form:
In 1X PBS, pH 7.4, with 0.09% sodium azide
Antibody Type:
Primary Antibody
Application Dilute:
Western blot: 1:100-1:500
Application Notes:
Titration of the GST antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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