The sequence cDYKDDDDK was used as the immunogen for this FLAG antibody.
Epitope tags are useful for the labeling and detection of recombinant proteins using western blotting, immunoprecipitation and immunostaining techniques. The eight amino acid DYKDDDDK peptide is an established and multi-functional epitope tag and can be expressed and detected with a recombinant protein as an amino-terminal or carboxy-terminal fusion.
Clonality:
Polyclonal
Purity:
Antigen affinity
Form:
In 1X PBS, pH 7.4, with 0.09% sodium azide
Antibody Type:
Primary Antibody
Application Dilute:
Western blot: 1:1000
Application Notes:
Titration of the FLAG antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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