HHHHHH peptide was used as the immunogen for the anti-His Tag antibody.
Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibodies have been widely used in the study of protein expression in various systems. 6xHIS-tagged fusion proteins are easily purified from cell lysates by affinity chromatography using Nickel-Sepharose resin.
Clonality:
Polyclonal
Purity:
Purified
Form:
In 1X PBS, pH 7.4, with 0.09% sodium azide
Antibody Type:
Primary Antibody
Application Dilute:
Western blot: 1:1000
Application Notes:
Titration of the anti-His Tag antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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