Poly-His peptide was used as the immungen for the His Tag antibody.
Epitope tags consisting of short sequences recognized by well-characterized monoclonal or polyclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH), recognized by the monoclonal or polyclonal antibody provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified by affinity chromatography using Nickel-Sepharose resin. NSJBios anti-6xHIS polyclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins.
Clonality:
Polyclonal
Purity:
Purified
Form:
In 1X PBS, pH 7.4, with 0.09% sodium azide
Antibody Type:
Primary Antibody
Application Dilute:
Western blot: 1:1000
Application Notes:
Titration of the His Tag antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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