A portion of amino acids 58-87 from the human protein was used as the immunogen for the TENT2 antibody.
GLD2 is cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3-end of specific RNAs, forming a poly(A) tail. In contrast to the canonical nuclear poly(A) RNA polymerase, it only adds poly(A) to selected cytoplasmic mRNAs. GLD2 does not play a role in replication-dependent histone mRNA degradation.
The stated application concentrations are suggested starting points. Titration of the TENT2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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