Recombinant human protein was used as the immunogen for the c-Myc antibody.
It recognizes a transcription factor of 64-67kDa, identified as c-myc. This mAb shows no cross-reaction with v-myc. c-Myc is a transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5-CAC[GA]TG-3. Activates the transcription of growth-related genes. [UniProt]
Prediluted in 1X PBS, 0.1 mg/ml BSA (US sourced), 0.05% sodium azide, *For IHC use only*
Antibody Type:
Primary Antibody
Application Dilute:
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Application Notes:
The stated application concentrations are suggested starting amounts. Optimal dilution of the c-Myc antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
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