A recombinant human HPV16 E1/E4 fragment (amino acids 36-41) was used as the immunogen for this HPV16 E1/E4 antibody.
The human papilloma virus (HPV) family of DNA tumor viruses includes HPV16, a high-risk sexually-transmitted HPV that can lead to cervical, anal, vulvar, head, neck, and penile cancer. The HPV16E1/E4 protein is expressed abundantly in cells supporting viral DNA amplification, but is lost during malignant progression. HPV16E1/E4 causes G2 cell cycle arrest by associating with and preventing the nuclear entry of Cdk1/cyclin B1 complexes. HPV16E1/E4 also interacts with cyclin A and Cdk2 during the G2 phase of the cell cycle, and this association may increase the efficiency with which HPV16E1/E4 is able to prevent mitotic entry. HPV16E1/E4 also associates with keratin intermediate filaments and causes the network to collapse.
Clonality:
Monoclonal
Clone Designation:
[HPV16 E1/E4]
Purity:
Protein G affinity chromatography
Form:
1 mg/ml in 1X PBS, BSA free, sodium azide free
Antibody Type:
Primary Antibody
Application Dilute:
Immunohistochemistry (FFPE): 1-2ug/ml
Application Notes:
Optimal dilution of the HPV16 E1/E4 antibody should be determined by the researcher.
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