Bromodeoxyuridine conjugated to KLH was used as the immunogen for the recombinant BrdU antibody.
It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
Clonality:
Monoclonal
Clone Designation:
[rBRD494]
UniProt:
Not Applicable
Purity:
Protein G affinity chromatography
Form:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Antibody Type:
Primary Antibody
Application Dilute:
Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
Application Notes:
Optimal dilution of the recombinant BrdU antibody should be determined by the researcher.
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