Immunogen was recombinant protein. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the immunogen. The IgG fraction of tissue culture supernatant was purified by Protein G/A affinity chromatography.
Mouse anti-Recombinant Staphylococcus aureus Penicillin Binding Protein 2 (PBP2a) Biotinylated Antibody, 50 ug
ELISA (recommended work dilution= 1:160,000), Western Blotting (recommended work dilution= 1:1,000-5,000),see image) Western Blot: First Antibody: 1µ,g/ml (Mouse anti-PBP2a (1X1000)), Second Antibody: 12,000X (HRP-Goat Anti-Mouse IgG) M: Protein Marker Lane 1: Recombinant PBP2a 1µ,g Lane 2: MSSA lysate 15µ,l
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