Recombinant Human Troponin T type 2 (cardiac), E.coli-derived
Molecular Weight:
The predicted molecular weight is 36.5 kDa. The actual recombinant protein migrates as approximately 45 kDa protein band in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions.
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Form:
Lyophilized powder
Sequence:
Ser2-Lys298
Formula:
Lyophilized from a 0.2 µm filtered solution in 0.1 M NaHCO3, 0.5 M NaCl, pH 7.5. Protein concentration prior to lyophilization was 0.25 mg/mL.
Application Notes:
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.1~ 1 mg/mL with sterile ice-cold deionized water or 1X Phosphate Buffered Saline (PBS). This solution can then be further diluted into other aqueous buffers.
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